期刊
BIOSENSORS & BIOELECTRONICS
卷 79, 期 -, 页码 467-475出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.087
关键词
Carbon dot; Tender coconut water; Microwave-hydrothermal; Photoluminescence; Thiamine sensor; Bioimaging
In this study microwave-assisted hydrothermal method was used to prepare highly luminescent carbon dots (1-6 nm size) within a minute from tender coconut (Cocos nucifera) water. The synthesized carbon dots (C-dots) exhibit emission of blue and green lights while excited at 390 and 450 nm wavelengths, respectively. As an application, these C-dots were tested for a simple turn on fluorescent sensor for rapid detection of thiamine (vitamin B-1). The detection of thiamine in human body is very important to prevent various diseases such as beriberi, neurological disorders, optic neuropathy, etc. The fluorescence emission intensity of C-dots quenches after addition of Cu2+ ion and then again increases selectively (turn on) after the addition of thiamine. The fluorescence emission intensity enhancement of Cu2+ ion modified C-dots in the presence of thiamine exhibits a linear relationship within the thiamine concentration range of 10-50 mu M. The limit of detection was found to be 280 nM from this study. The selectivity of the detection was also tested in the presence of different organic molecules and inorganic ions (Ca2+, Mg2+ Na+, K+, Cl-, SO42- and NO3-) which are present in blood serum and urine and found to be almost no interference in the detection. Finally, to see the applicability in real samples a commercial vitamin capsule was tested and found less than 3% error in the detected concentration. The C-dots were also used for bioimaging of fungus and the results show they are also suitable for this application too. (C) 2015 Elsevier B.V. All rights reserved.
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