期刊
BIOSENSORS & BIOELECTRONICS
卷 82, 期 -, 页码 233-239出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.04.003
关键词
Fluorescent pH sensor; Nitrogen-rich functional groups; Carbon nanoparticles; Live-cell image; Environmental monitoring
类别
资金
- National Natural Science Foundations of China [21405025, 21575031]
- Natural Science Foundations of Guangxi Province [2014GXNSFBA118047, 2015GXNSFDA139006]
- BAGUI Scholar Program
A nitrogen-rich functional groups carbon nanoparticles (N-CNs) based fluorescent pH sensor with a broad-range responding was prepared by one-pot hydrothermal treatment of melamine and triethanolamine. The as-prepared N-CNs exhibited excellent photoluminesence properties with an absolute quantum yield (QY) of 11.0%. Furthermore, the N-CNs possessed a broad-range pH response. The linear pH response range was 3.0 to 12.0, which is much wider than that of previously reported fluorescent pH sensors. The possible mechanism for the pH-sensitive response of the N-CNs was ascribed to photo induced electron transfer (PET). Cell toxicity experiment showed that the as-prepared N-CNs exhibited low cytotoxicity and excellent biocompatibility with the cell viabilities of more than 87%. The proposed N-CNs-based pH sensor was used for pH monitoring of environmental water samples, and pH fluorescence imaging of live T24 cells. The N-CNs is promising as a convenient and general fluorescent pH sensor for environmental monitoring and bioimaging applications. (C) 2016 Elsevier B.V. All rights reserved.
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