期刊
BIOSENSORS & BIOELECTRONICS
卷 81, 期 -, 页码 125-130出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.02.062
关键词
Optical aptasensor; Perylene probe; Aptamer; OTA; Hybridization chain reaction
类别
资金
- Southwest University (Chongqing, China) [SWU111071]
- Southwest University [SWU113111, SWU113112]
- National Natural Science Foundation of China [21505108]
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Wuhan University [ACBM2014004]
An optical aptasensor was developed for ultrasensitive detection of ochratoxin A (OTA) based on hybridization chain reaction (HCR) amplification strategy and fluorescent perylene probe (PAPDI)/DNA composites. Dendritic DNA concatamers were synthesized by HCR strategy and modified on magnetic nanoparticles through aptamer as medium. A large amount of PAPDI probe aggregated under the induction of DNA concatamers and caused fluorescence quenching. In the presence of OTA, the PAPDI/DNA composites were released from magnetic nanoparticles due to the strong affinity between aptamer and OTA. In ethanol, PAPDI monomers disaggregated and produced strong fluorescence. The present method displays excellent sensitivity and selectivity towards OTA. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据