期刊
BIOSENSORS & BIOELECTRONICS
卷 84, 期 -, 页码 133-140出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.079
关键词
D-penicillamine; Mn2+ quantum dots; Ratiometric sensing; Intracellular pH; Single fluorophore
类别
资金
- Ministry of Science and Technology (MOST) of Taiwan [MOST 103-2113-M-001-008-MY3]
- Academia Sinica Research Project on Nano Science and Technology [AS-104-TP-A11]
- MOST [NSC-102-2113-M-001-019-MY3]
We present a novel ratiometric pH sensor design using water-soluble, dual-mission, Mn2+-doped quantum dots (Qdots) decorated with D-penicillamine (DPA-MnQdots). In contrast to more commonly used ratiometric pH sensors that rely on the coupling of two fluorophores, our design uses only a single emitter, which simplifies ratiometric sensing and broadens the applications of the sensor. Our single emitter DPA-MnQdots exhibit two emission bands, at 510 nm (green) and 610 nm (red), which are, respectively, attributable to exciton recombination and emission of the Mn2+ dopants. The emission intensity ratio (I-510/I-610) of the DPA-MnQdots depends linearly on surrounding pH values within physiological conditions (from pH 4.5 to 8.5). Moreover, the biocompatible DPA-MnQdots were used for long-term monitoring of local pH values in HeLa cells. (C) 2016 Elsevier B.V. All rights reserved.
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