期刊
BIOSENSORS & BIOELECTRONICS
卷 81, 期 -, 页码 349-357出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.03.007
关键词
MWNT-GNP/Chi/MGCE; MNPs; Ana-1 cells; LPS Of pathogenic bacteria; EIS
类别
资金
- National Natural Science Foundation of China [31371768]
- NSFC-Guangdong province of China [U13012141]
- National Research Program [JUSRP51501, 201203069-1]
- Program for New Century Excellent Talents in Jiangnan University, QinglanProject [NCET-12-0877]
- Synergetic Innovation Center of Food Safety and Quality Control
- Priority Academic Program Development of Jiangsu Higher Education Institutions
In this study, a sensitive and simple electrochemical murine macrophage (Ana-1) cell sensor has been developed for early detection of lipopolysaccharides (LPS) to evaluate the toxicity of pathogenic bacteria. Magnetic glassy carbon electrode (MGCE), which possesses excellent reproducibility and regeneration qualities, was modified with a nanocomposite to improve electrochemical signals and enhance the sensitivity. The synthesized magnetic nanoparticles (MNPs) were internalized into murine macrophages, which completed the immobilization of macrophages onto the modified electrode for evaluating the cytotoxicity of LPS by electrochemical impedance spectroscopy (EIS). The MNPs facilitated reusability of the proposed sensor by allowing removal of the magnetic core from the electrode. Our results indicated that LPS caused a marked decrease in electrochemical impedance in a dose-dependent manner in range of 1-5 mu g/mL. By SEM, we found that microvilli on the plasma membrane became scarce and the membrane became smooth on cells incubated with LPS, which lessens the absorption of cells to reduce the impedance. And biological assay indicated that EIS patterns were correlated with the calcium concentration in cells, and suggested that [Ca2+](i) production increased in cells incubated with LPS and its mobilization altered electrochemical signals. Compared with conventional methods, this electrochemical test is inexpensive, highly sensitive, and has a quick response, and thus provides a new avenue for evaluating the cytotoxicity of pathogens. (C) 2016 Elsevier B.V. All rights reserved.
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