期刊
BIOSENSORS & BIOELECTRONICS
卷 85, 期 -, 页码 212-219出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.04.102
关键词
Electrochemical immunosensor; Prostate-specific antigen; Nanogold-decorated g-C3N4 nanosheets; Enzymatic biocatalytic precipitation
类别
资金
- National Natural Science Foundation of China [81402617]
This work reports on a new impedimetric immunosensing strategy for sensitive detection of prostate specific antigen (PSA) in biological fluids. The assay was carried out on monoclonal anti-PSA capture antibody-modified glassy carbon electrode with a sandwich-type detection format. Gold nanoparticles-decorated g-C3N4 nanosheets (AuNP/g-C3N4), synthesized by the wet-chemistry method, were utilized for the labeling of polyclonal anti-PSA detection antibody and horseradish peroxidase (HRP). Upon target PSA introduction, the sandwiched immunocomplex could be formed between capture antibody and detection antibody. Followed by the AuNP/g-C3N4, the labeled HRP could catalyze 4-choloro-1-naphthol into benzo-4-chlorohexadienone. The as-generated insoluble product was coated on the electrode surface, thus increasing the Faradaic impedance of Fe(CN)(6)(4-/3-) indicator between the solution and the base electrode. Under the optimal conditions, the impedance increased with the increasing target PSA in the sample, and exhibited a wide linear range from 10 pg mI(.-)1 and 30 ng mL(-1) with a detection limit of 5.2 pg mL(-1). A repeatability and intermediate precision of < 14% was accomplished. The specificity and method accuracy in comparison with commercial PSA ELISA kit for analysis of human serum specimens were relatively satisfactory. (C) 2016 Elsevier B.V. All rights reserved.
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