期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 80, 期 9, 页码 1768-1775出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2016.1158631
关键词
alcohol fermentation; branched-chain amino acid; stress; thiazole synthesis
类别
资金
- Bio-oriented Technology Research Advancement Institution
- Ministry of Education, Science, Culture and Sports, Japan
Thiamine pyrophosphate (TPP) is a critical cofactor and its biosynthesis is under the control of TPP availability. Here we disrupted a predicted thiA gene of the fungus Aspergillus nidulans and demonstrated that it is essential for synthesizing cellular thiamine. The thiamine riboswitch is a post-transcriptional mechanism for TPP to repress gene expression and it is located on A. nidulans thiA pre-messenger RNA. The thiA riboswitch was not fully derepressed under thiamine-limited conditions, and fully derepressed under environmental stressors. Upon exposure to hypoxic stress, the fungus accumulated more ThiA and NmtA proteins, and more thiamine than under aerobic conditions. The thiA gene was required for the fungus to upregulate hypoxic branched-chain amino acids and ethanol fermentation that involve enzymes containing TPP. These findings indicate that hypoxia modulates thiA expression through the thiamine riboswitch, and alters cellular fermentation mechanisms by regulating the activity of the TPP enzymes.
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