期刊
PROTOPLASMA
卷 257, 期 2, 页码 607-611出版社
SPRINGER WIEN
DOI: 10.1007/s00709-019-01447-2
关键词
Coccomyxa subellipsoidea C-169; Hygromycin B resistance; Electrotransformation; Electroporation; Stable genome transformation
In this study, we have shown the applicability of electroporation and hygromycin B as a convenient selectable marker for stable nuclear transformation of Coccomyxa subellipsoidea C-169. Since it is the first sequenced eukaryotic microorganism from polar environment, this offers unique opportunities to study adaptation mechanisms to cold.
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