期刊
PROCESS BIOCHEMISTRY
卷 90, 期 -, 页码 66-80出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2019.11.026
关键词
Enzyme immobilization; Facts and artifacts; Yield determination; Enzyme inactivation; Enzyme stabilization
资金
- [CTQ2017-86170R]
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors, as well as potential recycle. Whilst much has been reported in the scientific literature about enzyme immobilization methods, in many cases the protocol leads to losses in enzyme activity. In this review we outline the reasons for loss of activity during immobilization and highlight suitable diagnostic tests to elucidate the precise cause and thereby methods to restore activity. The need for standardized reporting of immobilization methods is also emphasized as a means of benchmarking alternative approaches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据