期刊
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
卷 50, 期 2, 页码 191-197出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2019.1692215
关键词
Gellan gum; CSL; cost-effective; Plackett-Burman design; Box-Behnken
类别
资金
- Yunhe Program of Zhejiang University of Technology
- General Project of Beijing Municipal Education Commission [SQKM201311417004]
- Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, P.R. China [ZXKF20180204]
Gellan gum, produced by Sphingomonas paucimobilis, is increasingly used in food and pharmaceutical industries as stabilizing, emulsifying, texturing and gelling agents. However, its high production costs may limit its full commercial potential. Therefore, in this study, we investigated ways to reduce gellan gum production costs and improve yields. We first revealed corn steep liquor (CSL) as a cost-effective nutrient source that can improve gellan gum yields. We then systematically optimized culture conditions even further, and revealed that the addition of Triton X-100 surfactant and selected inorganic nitrogen sources improved gellan gum production. Under our optimized conditions (glucose 33.75 g/L, CSL 10 g/L, urea 2.5 g/L, MgSO4 1.08 g/L, KH2PO4 3.24 g/L, K2SO4 1 g/L and Triton X-100 0.75 g/L), we yielded a maximum concentration of 14.41 g/L, which was about 1.5-fold higher than non-optimized CSL-based medium. Our findings highlight the use of CSL as a cost effective and promising nutrient source for industrial production of gellan gum.
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