期刊
POLISH JOURNAL OF ENVIRONMENTAL STUDIES
卷 29, 期 2, 页码 1077-1086出版社
HARD
DOI: 10.15244/pjoes/104660
关键词
ecological risk; single-cell gel electrophoresis; heavy metal; DNA damage; 7-ethoxyresorufin-O-deethylase
资金
- Basic Environmental Research Program of the Geum River System, (NIER-2018)
- Ministry of the Environment, Korea
- Daejeon Green Environment Center under the Research Development Program (Year 2017)
Ecological risk assessment was carried out in two urban streams, viz. Gap Stream (GS) and Miho Stream (MS), in the Geum River Watershed during July 2013-October 2014. The techniques used in this study included multi-level fish biomarkers of DNA damage based on single-cell gel electrophoresis (SCGE) coupled with the study of physiological responses based on 7-ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities in fish species. Biomarker values of tail DNA (tDNA), tail length (T-L), and tail extent moment (TEM) in impacted zone (I-z) were 2.0-3.6-fold greater than in controls (C-z). Nucleus image analysis showed that the nucleus had circular particle forms in the C-z as compared with a longitudinal oval form with broken particles from the nucleus in the I-z. Physiological response analysis of EROD and AchE activities indicated that their levels were higher in the I-z than in the C-z. Such DNA damages and greater physiological responses in the I-z were attributed to chemical contaminants discharged from the wastewater disposal plants and industrial complex. This combination of DNA damage and physiological responses approach can be used as a key tool for early-warning detection of chemical contaminants and concomitant risks to the ecological health of urban streams.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据