期刊
PLANT SCIENCE
卷 287, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2019.110176
关键词
Ammonium; Auxin; Indole-3-acetaldoxime; Indole-3-acetaldehyde oxidase; Nitrate; Oximes; Phenotype; Superroot; Root; Urea; CYP71A
资金
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2017-86293-P, CGL2017-84723-P]
- Ministry of Science Innovation and Universities (MICINN) [AGL2014-52396-P]
- Basque Government, Spain [IT932-16]
- Public University of Navarre
- Basque Government through the BERC 2018-2021 program
- Spanish Ministry of Science, Innovation and Universities through the BC3 Maria de Maeztu excellence accreditation [MDM-2017-0714]
- Juan de la Cierva initiation grant [FJCI-2016-27905]
- Juan de la Cierva incorporation grant [IJCI-2014-21452]
Indole-3-acetaldoxime (IAOx) is a particularly relevant molecule as an intermediate in the pathway for tryptophan-dependent auxin biosynthesis. The role of IAOx in growth-signalling and root phenotype is poorly studied in cruciferous plants and mostly unknown in non-cruciferous plants. We synthesized IAOx and applied it to M. truncatula plants grown axenically with NO3-, NH4+ or urea as the sole nitrogen source. During 14 days of growth, we demonstrated that IAOx induced an increase in the number of lateral roots, especially under NH4+ nutrition, while elongation of the main root was inhibited. This phenotype is similar to the phenotype known as superroot previously described in SUR1- and SUR2-defective Arabidopsis mutants. The effect of IAOx, IAA or the combination of both on the root phenotype was different and dependent on the type of N-nutrition. Our results also showed the endogenous importance of IAOx in a legume plant in relation to IAA metabolism, and suggested IAOx long-distance transport depending on the nitrogen source provided. Finally, our results point out to CYP71A as the major responsible enzymes for IAA synthesis from IAOx, while they exclude indole-3-acetaldehyde oxidases.
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