期刊
PLANT AND CELL PHYSIOLOGY
卷 61, 期 1, 页码 158-168出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcz188
关键词
Acyltransferase; Alginate gel; Chlamydomonas; Gel encapsulation; Palmelloid colony; Triacylglycerol
资金
- Yashima Environmental Technology Foundation
- foundation for the interaction of Science & Technology, Japan
- Japan Society for the Promotion of Science KAKENHI [18K04845]
- Japan Science and Technology Agency
- [17H03715]
- Grants-in-Aid for Scientific Research [18K04845] Funding Source: KAKEN
Microalgae such as Chlamydomonas reinhardtii accumulate triacylglycerol (TAG), which is a potential source of biofuels, under stress conditions such as nitrogen deprivation, whereas Chlamydomonas debaryana NIES-2212 has previously been identified and characterized as one of the rare species of Chlamydomonas, which massively accumulates TAG in the stationary phase without external stress. As the high density of the cells in the stationary phase was supposed to act as a trigger for the accumulation of TAG in C. debaryana, in this study, C. debaryana was encapsulated in a Ca2+-alginate gel for the culture with high cell density. We discovered that the growth of the encapsulated cells resulted in the formation of spherical palmelloid colonies with high cell density, where daughter cells with truncated flagella remained wrapped within the mother cell walls. Interestingly, gel encapsulation markedly promoted proliferation of C. debaryana cells, and the encapsulated cells reached the stationary phase earlier than that of the freeliving cells. Gel encapsulation also enhanced TAG accumulation. Gene expression analysis revealed that two genes of acyltransferases, DGAT1 and DGTT3, were upregulated in the stationary phase of free-living C. debaryana. In addition, the gene expression of these acyltransferases increased earlier in the encapsulated cells than that in the free-living cells. The enhanced production of TAG by alginate gel encapsulation was not found in C. reinhardtii which is known to use a different repertoire of acyltransferases in lipid accumulation.
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