期刊
PHYTOMEDICINE
卷 64, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2019.153054
关键词
PSORI-CM02; Psoriasis; Autophagy; PI3K/Akt/mTOR
资金
- National Natural Science Foundation of China [81803804]
- Guangdong Province Natural Science Fund [S2013030011515, 2017A030310124]
- Guangdong Province Science and Technology Planning Project [2017A050506041, 2017B030314166]
- Guangdong Provincial Department of Education Project [2018KQNCX046]
- Guangzhou Science and Technology Project [201807010051]
- Guangdong Provincial Hospital of Chinese Medicine Special Fund [YN2016ZD01, YN2018HK01, YN2018ZD08, YN2018RBA02, YN0216XP02]
Background: Psoriasis is an inflammatory skin disease that affects an estimated 3% of the world's population. PSORI-CM02 is an empirically developed Chinese medicine formula optimised from Yin Xie Ling, summarised by national medical master Guo-Wei Xuan, that has been used for decades to treat psoriasis in the Guangdong Provincial Hospital of Chinese Medicine. However, its anti-psoriatic mechanisms are still poorly understood. In this study, we explored the effects of PSORI-CM02 on autophagy and the underlying mechanisms in TNF-alpha-stimulated HaCaT cells and in a mouse model of imiquimod-induced psoriasis. Methods: Cell viability was assessed by MTT assay. Apoptosis was detected by annexin V-FITC/PI double-staining and caspase-3 assays. Autophagy was detected by electron microscopy, RT-PCR and western blotting. The PI3K/Akt/mTOR pathway was analysed by western blotting and immunochemical analysis. Results: PSORI-CM02 induced autophagy and thus inhibited the proliferation of HaCaT cells via suppression of the PI3K/Akt/mTOR pathway. In mice with IMQ-induced psoriasis, PSORI-CM02 relieved psoriasis symptoms, induced autophagy and inhibited the phosphorylation of the PI3K/AKT/mTOR pathway in the skin. Conclusion: These results suggest that PSORI-CM02 treats psoriasis by inducing autophagy via inhibition of the PI3K/Akt/mTOR pathway.
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