期刊
PHYTOMEDICINE
卷 64, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2019.153065
关键词
Arnicolide D; Melanoma; Cell cycle arrest; Apoptosis; NF-kappa B
资金
- National Natural Science Foundation of China [81673649, 8187141799, 81803788, 81872769]
- Research Grants Council of Hong Kong [12125116, 12102918]
- Food and Health Bureau of Hong Kong [14150571, 15163441]
- Shenzhen Science and Technology Innovation Committee [JCYJ20151030164022389, JCYJ20160229173844278, JCYJ20160229210327924, JCYJ20170817173608483]
- Guangdong Provincial Department of Science and Technology [2016A030313007]
- Hong Kong Baptist University [FRG1/1617/048, FRG2/17-18/032]
Background: Melanoma is a lethal cancer. NF-kappa B has been validated as a molecular target for melanoma treatment. Current therapies for melanoma have limitations. Novel targeted therapeutics are needed. Arnicolide D (Ar-D), a sesquiterpene lactone isolated from the dried whole plant of Centipeda minima (L.) A. Br. et Aschers., has been reported to inhibit NF-kappa B activity in colorectal cancer cells. Purpose: To investigate the anti-melanoma effects of Ar-D in vitro and in vivo; and to determine whether Ar-D inhibits the NF-kappa B pathway in melanoma cells. Methods: A B16F10 allograft mouse model and two melanoma cell lines (A375 and B16F10) were used to investigate the anti-melanoma effects of Ar-D in vivo and in vitro. Dacarbazine was used as a positive control. Cell viability was assessed by MTT and crystal violet staining assays. Cell cycle arrest and apoptosis were analyzed by flow cytometry. Protein levels were determined by Immunoblotting. Results: In vivo assays showed that the average tumor weight in Ar-D-treated group (4 mg/kg, i.p, 15 days) was reduced by 53.7%, when compared with the control group. In vitro studies demonstrated that Ar-D reduced cell viability, induced G2/M cell cycle arrest and apoptosis, elevated levels of cell cycle regulatory proteins p53 and p21, and lowered levels of G2/M checkpoint proteins Cdc2 and Cyclin B1 in melanoma cells. Mechanistically, Ar-D inhibited the activity of IKK alpha/beta, the degradation of I kappa B alpha, and the phosphorylation and expression of NF-kappa B p65 in melanoma cells. Conclusion: Ar-D has anti-melanoma effects, and inhibition of the IKK/I kappa B alpha/NF-kappa B p65 pathway is involved in the effects.
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