期刊
BIORESOURCE TECHNOLOGY
卷 204, 期 -, 页码 49-54出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.12.076
关键词
Haematococcus pluvialis; Nuclear mutagenesis; High CO2; Astaxanthin; Growth rate
资金
- National Natural Science Foundation-China [51176163, 51476141]
- Zhejiang Provincial Natural Science Foundation-China [LR14E060002]
Unicellular green microalgae Haematococcus pluvialis was mutated with Co-60-gamma irradiation to promote growth rate and increase astaxanthin yield under high concentration of CO2 stress. The average specific growth rate of H. pluvialis mutated with 4000 Gy gamma-ray irradiation was increased by 15% compared with the original strain with air aeration. The mutant grew best with 6% CO2 (the maximum specific growth rate was 0.60/d) when it was cultured with high concentrations of CO2 (2-10%). The peak biomass productivity (0.16 g/L/d) of the mutant cultured with 6% CO2 was 82% higher than that of the mutant with air. The astaxanthin yield and lipid content of the mutant induced with 6% CO2 and high light (108 mu mol photons m(-2) s(-1)) increased to 46.0 mg/L and 45.9%, which were 2.4 and 1.3 times higher than those of the wild-type strain, respectively. (C) 2016 Elsevier Ltd. All rights reserved.
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