期刊
BIORESOURCE TECHNOLOGY
卷 215, 期 -, 页码 324-333出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.03.132
关键词
Cell-surface display engineering; Waste biorefinery; Biofuel; Biochemical; Heavy metal; Yeast
资金
- State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology, China) [2016TS07]
Waste biorefinery aims to maximize the output of value-added products from various artificial/agricultural wastes by using integrated bioprocesses. To make waste biorefinery economically feasible, it is thus necessary to develop a low-cost, environment-friendly technique to perform simultaneous biodegradation and bioconversion of waste materials. Cell-surface display engineering is a novel, cost-effective technique that can auto-immobilize proteins on the cell exterior of microorganisms, and has been applied for use with waste biofinery. Through tethering different enzymes (e.g., cellulase, lipase, and protease) or metal-binding peptides on cell surfaces, various yeast strains can effectively produce biofuels and biochemicals from sugar/protein-rich waste materials, catalyze waste oils into biodiesels, or retrieve heavy metals from wastewater. This review critically summarizes recent applications of yeast cell-surface display on various types of waste biorefineries, highlighting its potential and future challenges with regard to commercializing this technology. (C) 2016 Elsevier Ltd. All rights reserved.
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