期刊
BIORESOURCE TECHNOLOGY
卷 200, 期 -, 页码 744-752出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.10.078
关键词
Illumina sequencing; Metagenomics; Microbiome; 16S rRNA gene; Succession
资金
- Department of Energy (DOE) award [DE-FG36-05GO85010]
- United States Department of Agriculture/National Institute of Food and Agriculture (USDA/NIFA) award [2012-10008-20302]
The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据