Phagocytic activity of human macrophages and Drosophila hemocytes after exposure to the neonicotinoid imidacloprid

Neonicotinoid insecticides are increasingly used in modern pest control and in conventional agriculture. Their residues are frequently found in our environment and in our food leading to chronic exposure of pollinating insects and humans. Indeed, evidence has become stronger that chronic exposure to neonicotinoids might have a direct impact on the immune response of invertebrates and vertebrates. Therefore, we compared the cellular immune response of human macrophages (THP-1) and Drosophila melanogaster hemocytes (Schneider 2 cells) after exposure to four different concentrations of the neonicotinoid imidacloprid. Cells were immune activated with LPS (lipopolysaccharide) of Escherichia coli to compare the phagocytic activity of immune activated and non-activated cells during pesticide exposure. Drosophila cells were more strongly affected by the insecticide than human macrophages. Even though imidacloprid showed an adverse effect on phagocytosis on both cells while immune activated, it decreased phagocytosis in Drosophila cells at shorter exposure time and without immune activation.