期刊
BIORESOURCE TECHNOLOGY
卷 222, 期 -, 页码 195-201出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.10.006
关键词
Biodegradation; 2,6-Dichlorophenol indophenol (DCPIP); Gas chromatography; Petroleum hydrocarbons; P. aeruginosa NCIM 5514
The aim of this work was to study the potential of an indigenous strain of Pseudomonas aeruginosa NCIM 5514, isolated from petroleum-polluted soil, for the biodegradation of crude petroleum oil. The isolate completely decolorized 2,6-dichlorophenol indophenol in 120 h when grown at (37 +/- 1 degrees C), indicating its hydrocarbon utilizing nature. Ex situ biodegradation study was performed to find out quantitative utilization and biodegradation of paraffin(s) present in crude oil. When the culture was grown in Bushnell-Hass medium containing crude oil (3%, v/v) at 37 degrees C, 180 rpm for 60 days, the viscosity of the oil was reduced from 1883 cp to 1002 cp. Gravimetric and gas chromatographic analysis showed 61.03% and 60.63% of biodegradation of C8-C36+ hydrocarbons, respectively. These results indicated that the isolate has potential to be used for ex-situ and in-situ bioremediation of hydrocarbon pollutants and could have promising applications in petrochemical industry. (C) 2016 Elsevier Ltd. All rights reserved.
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