期刊
BIORESOURCE TECHNOLOGY
卷 216, 期 -, 页码 637-644出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.06.004
关键词
Recycled paper sludge; Cellulase recycling; Ultrafiltration; Alkaline wash; Cellulosic bioethanol
资金
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469/2013]
- COMPETE 2020 [POCI-01-0145-FEDER-006684]
- FCT through GlycoCBMs Project [PTDC/AGR-F OR/3090/2012-FCOMP-01-0124-FEDER-027948, SFRH/BD/88623/2012]
- [RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462)]
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-FOR/3090/2012] Funding Source: FCT
The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge ( RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed: 71, 64 and 100% of the initial Cel7A, Cel7B and beta-glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs. (C) 2016 Elsevier Ltd. All rights reserved.
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