期刊
BIORESOURCE TECHNOLOGY
卷 200, 期 -, 页码 413-419出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.10.045
关键词
Bacillus; Biowaste; Co-polymer; Defined mixed culture; Volatile fatty acids
资金
- CSIR-Institute of Genomics and Integrative Biology, Delhi, India
- WUM [ESC0108]
- CSIR
Production of polyhydroxyalkanoate (PHA) co-polymers by Bacillus spp. was studied by feeding defined volatile fatty acids (VFAs) obtained through controlled hydrolysis of various wastes. Eleven mixed hydrolytic cultures (MHCs) each containing 6 strains could generate VFA from slurries of (2% total solids): pea-shells (PS), potato peels (PP), apple pomace (AP) and onion peels (OP). PS hydrolysates (obtained with MHC2 and MHC5) inoculated with Bacillus cereus EGU43 and Bacillus thuringiensis EGU45 produced co-polymers of PHA at the rate of 15-60 mg/L with a 3HV content of 1% w/w. An enhancement in PHA yield of 3.66-fold, i.e. 205-550 mg/L with 3HV content up to 7.5% (w/w) was observed upon addition of OP hydrolysate and 1% glucose (w/v) to PS hydrolysates. This is the first demonstration, where PHA co-polymer composition, under non-axenic conditions, could be controlled by customizing VFA profile of the hydrolysate by the addition of different biowastes. (C) 2015 Elsevier Ltd. All rights reserved.
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