期刊
NEW PHYTOLOGIST
卷 225, 期 2, 页码 807-822出版社
WILEY
DOI: 10.1111/nph.16161
关键词
alpha integrin-like protein; defective pollen wall; male sterile; tapetum degradation; Ubisch body
资金
- National Key Research and Development Program of China [2016YFD0100804, 2016YFE0101000]
- National Natural Science Foundation of China [31430009]
- NSFC projects of International Cooperation and Exchanges [31861163002]
- China Postdoctoral Science Foundation [17Z102060083]
- 111 Project [B14016]
- Science and Technology Commission of Shanghai Municipality [13JC1408200]
- Australia-China Science and Research Fund Joint Research Centre [ACSRF48187]
- National Transgenic Major Program [2016ZX08009-003007, 2016ZX08012002]
- China scholarship council (CSC) [2012GXZ494]
- Innovative Research Team, Ministry of Education
In flowering plants, pollen wall is a specialized extracellular cell-wall matrix surrounding male gametophytes and acts as a natural protector of pollen grains against various environmental and biological stresses. The formation of pollen wall is a complex but well-regulated process, which involves the action of many different genes. However, the genetic and molecular mechanisms underlying this process remain largely unknown. In this study, we isolated and characterized a novel rice male sterile mutant, defective pollen wall3 (dpw3), which displays smaller and paler anthers with aborted pollen grains. DPW3 encodes a novel membrane-associated alpha integrin-like protein conserved in land plants. DPW3 is ubiquitously expressed in anther developmental stages and its protein is localized to the plasma membrane, endoplasmic reticulum (ER) and Golgi. Anthers of dpw3 plants exhibited unbalanced anther cuticular profile, abnormal Ubisch bodies, disrupted callose deposition, defective pollen wall formation such as abnormal microspore plasma membrane undulation and defective primexine formation, resulting in pollen abortion and complete male sterility. Our findings revealed a novel and vital role of alpha integrin-like proteins in plant male reproduction.
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