期刊
BIORESOURCE TECHNOLOGY
卷 199, 期 -, 页码 121-127出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.09.049
关键词
Weak acid; Weak alkali; Acetic acid hydrothermal (AAH); Pretreatment; Enzymatic digestibility
资金
- R&D program of MOTIE/KEIT [10049674]
Fractionation of EFB was conducted in two consecutive steps using a batch reaction system: hemicellulose hydrolysis using acetic acid (AA; 3.0-7.0 wt.%) at 170-190 degrees C for 10-20 min in the first stage, and lignin solubilization using ammonium hydroxide (5-20 wt.%) at 140-220 degrees C for 5-25 min in the second stage. The two-stage process effectively fractionated empty fruit bunches (EFB) in terms of hemicellulose hydrolysis (53.6%) and lignin removal (59.5%). After the two-stage treatment, the fractionated solid contained 65.3% glucan. Among three investigated process parameters, reaction temperature and ammonia concentration had greater impact on the delignification reaction in the second stage than reaction time. The two-stage fractionation processing improved the enzymatic digestibility to 72.9% with 15 FPU of cellulase/g of glucan supplemented with 70 pNPG of beta-glycosidase (Novozyme 188)/g-glucan, which was significantly enhanced from the equivalent digestibility of 28.3% for untreated EFB and 45.7% for AAH-fractionated solid. (C) 2015 Elsevier Ltd. All rights reserved.
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