期刊
NATURE CHEMISTRY
卷 12, 期 1, 页码 48-55出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41557-019-0366-y
关键词
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资金
- Ontario Graduate Scholarship
- NSERC [RGPIN-2016-06352]
- CIHR Foundation Grant Program [201610FDN-375469]
- University of Toronto
- CIHR Canada Research Chair Program [950-231075]
- University of Toronto's Medicine by Design initiative - Canada First Research Excellence Fund [C1TPA-2016-06]
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R21AI136571]
- NIH Director's New Innovator Award [1DP2GM126892]
- Arizona Biomedical Research Commission New Investigator Award [ADHS16-162400]
- Alfred P. Sloan Research Fellowship [FG-2017-9108]
- Gates Foundation funds [OPP1160667]
- Gordon and Betty Moore Foundation [6984]
- Bill and Melinda Gates Foundation [OPP1160667] Funding Source: Bill and Melinda Gates Foundation
The field of synthetic biology has used the engineered assembly of synthetic gene networks to create a wide range of functions in biological systems. To date, gene-circuit-based sensors have primarily used optical proteins (for example, fluorescent, colorimetric) as reporter outputs, which has limited the potential to measure multiple distinct signals. Here we present an electrochemical interface that permits expanded multiplexed reporting for cell-free gene-circuit-based sensors. We have engineered a scalable system of reporter enzymes that cleave specific DNA sequences in solution, which results in an electrochemical signal when these newly liberated strands are captured at the surface of a nanostructured microelectrode. We describe the development of this interface and show its utility using a ligand-inducible gene circuit and toehold switch-based sensors by demonstrating the detection of multiple antibiotic resistance genes in parallel. This technology has the potential to expand the field of synthetic biology by providing an interface for materials, hardware and software.
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