期刊
NATURE
卷 574, 期 7778, 页码 413-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41586-019-1641-1
关键词
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资金
- NIH [1DP1NS087724, 1R01GM104948, 1R01EB024261, 1R01DA045549, 1R01MH114031, 1R01NS102727, 1R43MH109332, 2R01DA029639]
- NSF [1734870, CBET-1848029, CBET 1344219]
- HHMI-Simons Faculty Scholars Program
- Human Frontier Science Program [RGP0015/2016]
- US Army Research Laboratory [W911NF1510548]
- US Army Research Office [W911NF1510548]
- NIH Director's Office [1DP2NS082126]
- NINDS [1R01NS109794-01, 1R01NS087950-01]
- Grainger Foundation
- Pew Foundation
- Boston University Biomedical Engineering Department
- NIH/NIGMS T32 Quantitative Biology and Physiology Fellowship through the Boston University Biomedical Engineering Department [GM008764]
- Direct For Education and Human Resources
- Division Of Undergraduate Education [1734870] Funding Source: National Science Foundation
A longstanding goal in neuroscience has been to image membrane voltage across a population of individual neurons in an awake, behaving mammal. Here we describe a genetically encoded fluorescent voltage indicator, SomArchon, which exhibits millisecond response times and is compatible with optogenetic control, and which increases the sensitivity, signal-to-noise ratio, and number of neurons observable several-fold over previously published fully genetically encoded reagents(1-8). Under conventional one-photon microscopy, SomArchon enables the routine population analysis of around 13 neurons at once, in multiple brain regions (cortex, hippocampus, and striatum) of head-fixed, awake, behaving mice. Using SomArchon, we detected both positive and negative responses of striatal neurons during movement, as previously reported by electrophysiology but not easily detected using modern calcium imaging techniques(9-11), highlighting the power of voltage imaging to reveal bidirectional modulation. We also examined how spikes relate to the subthreshold theta oscillations of individual hippocampal neurons, with SomArchon showing that the spikes of individual neurons are more phase-locked to their own subthreshold theta oscillations than to local field potential theta oscillations. Thus, SomArchon reports both spikes and subthreshold voltage dynamics in awake, behaving mice.
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