Anti-inflammatory effect and mechanism of action of ellagic acid-3,3′,4-trimethoxy-4′-O-α-L-rhamnopyranoside isolated from Hopea parviflora in lipopolysaccharide-stimulated RAW 264.7 macrophages

Phytochemical investigation of the stem bark of Hopea parviflora resulted in the isolation of 9 compounds; which includes friedelin (1), friedelin-3 beta-ol (2), (-)-ampelopsin A (3), (-)--viniferin (4), (-)-hopeaphenol (5), vaticaphenol A (6), 2,4,8-trihydroxyphenanthrene-2-O-glucoside (7), ellagic acid-3,3',4-trimethoxy-4'-O-alpha-L-rhamnopyranoside (8) and beta-sitosterol-beta-D-glucoside (9). Among them, compounds 1, 2, 6, 7, 8 and 9 are isolated for the first time from this species. Further, we evaluated the anti-inflammatory activity of compounds 4, 5, 6, 7 and 8. In this study, compound 8 inhibited the activity of proinflammatory mediators like NO, TNF-alpha, IL-6, 5-LOX and COX-2, also promoted the action of anti-inflammatory mediator like IL-10 via inhibition of the NF-kappa B pathway in LPS-stimulated RAW 264.7 macrophages. [GRAPHICS] .

Automated summary

Phytochemical investigation of the stem bark of Hopea parviflora led to the isolation of 9 compounds, with 6 of them being isolated from this species for the first time. Compound 8 showed anti-inflammatory activity by inhibiting proinflammatory mediators and promoting anti-inflammatory mediators.