期刊
NANO LETTERS
卷 19, 期 11, 页码 7645-7654出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b02242
关键词
Copper/Carbon nanozymes; copper state-dependent; releasing Cu2+; ROS; antibacterial therapy
类别
资金
- National Natural Science Foundation of China [21703198, 81671810]
- University Natural Science Foundation of Jiangsu Province [16KJD150004]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Top-Notch Academic Programs Project of Jiangsu Higher Education Institutions
Metal-carbon hybrid materials have shown promise as potential enzyme mimetics for antibacterial therapy; however, the effects of metal states and corresponding antibacterial mechanisms are largely unknown. Here, two kinds of copper/carbon nanozymes were designed, with tuned copper states from Cu-0 to Cu2+. Results revealed that the copper/carbon nanozymes exhibited copper state-dependent peroxidase-, catalase-, and superoxide dismutase-like activities. Furthermore, the antibacterial activities were also primarily determined by the copper state. The different antibacterial mechanisms of these two copper/carbon nanozymes were also proposed. For the CuO-modified copper/carbon nanozymes, the released Cu2+ caused membrane damage, lipid peroxidation, and DNA degradation of Gram-negative bacteria, whereas, for Cu-modified copper/carbon nanozymes, the generation of reactive oxygen species (ROS) via peroxidase-like catalytic reactions was the determining factor against both Gram-positive and Gram-negative bacteria. Lastly, we established two bacterially infected animal models, i.e., bacteria-infected enteritis and wound healing, to confirm the antibacterial ability of the copper/carbon nanozymes. Our findings provide a deeper understanding of metal state-dependent enzyme-like and antibacterial activities and highlight a new approach for designing novel and selective antibacterial therapies based on metal-carbon nanozymes.
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