期刊
NANO LETTERS
卷 20, 期 1, 页码 101-108出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b03344
关键词
Gold nanoparticle; optical coherence tomography; contrast agent; multiplexing; tumor lymphatic system; in vivo imaging
类别
资金
- United States Air Force [FA9550-15-1-0007]
- National Institutes of Health [NIH DP50D012179]
- National Science Foundation [NSF 1438340]
- Damon Runyon Cancer Research Foundation (DFS) [06-13]
- Claire Giannini Fund
- Susan G. Komen Breast Cancer Foundation [SAB15-00003]
- Mary Kay Foundation [017-14]
- Skippy Frank Foundation
- Donald E. and Delia B. Baxter Foundation
- Center for Cancer Nanotechnology Excellence and Translation (GCNE-T) [NIH-NCI U54CA151459]
- Stanford Bio-X Interdisciplinary Initiative Seed Grant [IIP6-43]
- Pew Charitable Trusts
- Alexander and Margaret Stewart Trust
Developing contrast-enhanced optical coherence tomography (OCT) techniques is important for specific imaging of tissue lesions, molecular imaging, cell-tracking, and highly sensitive microangiography and lymphangiography. Multiplexed OCT imaging in the second near-infrared (NIR-II) window is highly desirable since it allows simultaneous imaging and tracking of multiple biological events in high resolution with deeper tissue penetration in vivo. Here we demonstrate that gold nanobipyramids can function as OCT multiplexing contrast agents, allowing high-resolution imaging of two separate lymphatic flows occurring simultaneously from different drainage basins into the same lymph node in a live mouse. Contrast-enhanced multiplexed lymphangiography of a melanoma tumor in vivo shows that the peritumoral lymph flow upstream of the tumor is unidirectional, and tumor is accessible to such flow. Whereas the lymphatic drainage coming out from the tumor is multidirectional. We also demonstrate real-time tracking of the contrast agents draining from a melanoma tumor specifically to the sentinel lymph node of the tumor and the three-dimensional distribution of the contrast agents in the lymph node.
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