期刊
MYCOSCIENCE
卷 61, 期 2, 页码 85-94出版社
ELSEVIER
DOI: 10.1016/j.myc.2019.10.003
关键词
Appressorium; Cochliobolus heterostrophus; Penetration; Plant pathogen
类别
资金
- JSPS KAKENHI [15K07311, 19K06052]
- Grants-in-Aid for Scientific Research [15K07311, 19K06052] Funding Source: KAKEN
A mitogen-activated protein kinase (MAPK) signaling pathway regulates specialized cellular responses to external stimuli. In Bipolaris maydis, a Chk1 MAPK orthologous to Fus3/Kss1 MAPKs of Saccharomyces cerevisiae is known to regulate various developmental processes, including the formation of appressoria. However, upstream factors that regulate the Chk1 cascade have not been well clarified. In this study, we identified and characterized the BmSte50 gene, an ortholog of the yeast Ste50 in B. maydis. Our yeast two-hybrid assay indicated that BmSte50 interacts with a MAPK kinase kinase BmSte11, a component of the Chk1 cascade. Delta BmSte50 strains exhibited a loss of pathogenicity due to a lack of appressorial formation. The mutants also showed a reduction in melanization, conidial production, and aerial-mycelial and sexual development. Such phenotypes of the mutants were consistent with those of the Chk1 cascade gene mutants previously reported. In addition, ?BmSte50 strains indicated lower conidial germination efficiency than the wild type. Notably, a significant number of Delta BmSte50 conidia could be germinated, while the Chk1 cascade gene mutants were reported to lack conidial germination ability. Our results suggested that Delta BmSte50 may act as an adaptor protein for the Chk1 cascade and is involved in the regulation of various cellular processes. (C) 2019 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据