期刊
MOLECULES
卷 24, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/molecules24224068
关键词
resins; limonene; triterpenes; microsamples; capillary liquid chromatography (Cap-LC)
资金
- EU FEDER
- Spanish Agencia Espanola de Investigacion, AEI [CTQ2017-90082-P]
- Generalitat Valenciana [PROMETEO 2016/109]
- Universidad Nacional Autonoma de Honduras (Honduras)
A method has been developed for the separation and quantification of terpenic compounds typically used as markers in the chemical characterization of resins based on capillary liquid chromatography coupled to UV detection. The sample treatment, separation and detection conditions have been optimized in order to analyze compounds of different polarities and volatilities in a single chromatographic run. The monoterpene limonene and the triterpenes lupeol, lupenone, beta -amyrin, and alpha -amyrin have been selected as model compounds. The proposed method provides linear responses and precision (expressed as relative standard deviations) of 0.6% to 17%, within the 0.5-10.0 mu g mL(-1) concentration interval; the limits of detection (LODs) and quantification (LOQs) were 0.1-0.25 mu g mL(-1) and 0.4-0.8 mu g mL(-1), respectively. The method has been applied to the quantification of the target compounds in microsamples. The reliability of the proposed conditions has been tested by analyzing three resins, white copal, copal in tears, and ocote tree resin. Percentages of the triterpenes in the range 0.010% to 0.16% were measured using sample amounts of 10-15 mg, whereas the most abundant compound limonene (>= 0.93%) could be determined using 1 mg portions of the resins. The proposed method can be considered complementary to existing protocols aimed at establishing the chemical fingerprint of these kinds of samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据