期刊
MOLECULES
卷 24, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/molecules24224134
关键词
DNAzyme activity; hairpin DNA; logic circuits; leakage
资金
- National Natural Science Foundation of China [61425002, 61751203, 61772100, 61672121, 61972266, 61802040, 61572093]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R07]
- Program for Liaoning Innovative Research Team in University [LT2017012]
- Natural Science Foundation of Liaoning Province [20180551241, 2019-ZD-0567]
- High-level Talent Innovation Support Program of Dalian City [2017RQ060, 2018RQ75]
- Dalian Outstanding Young Science and Technology Talent Support Program [2017RJ08]
- Scientific Research Starting Foundation of Shenyang Aerospace University [18YB38]
Recently, DNA molecules have been widely used to construct advanced logic devices due to their unique properties, such as a simple structure and predictable behavior. In fact, there are still many challenges in the process of building logic circuits. Among them, the scalability of the logic circuit and the elimination of the crosstalk of the cascade circuit have become the focus of research. Inspired by biological allosteric regulation, we developed a controllable molecular logic circuit strategy based on the activity of DNAzyme. The E6 DNAzyme sequence was temporarily blocked by hairpin DNA and activated under appropriate input trigger conditions. Using a substrate with ribonucleobase (rA) modification as the detection strand, a series of binary basic logic gates (YES, AND, and INHIBIT) were implemented on the computational component platform. At the same time, we demonstrate a parallel demultiplexer and two multi-level cascade circuits (YES-YES and YES-Three input AND (YES-TAND)). In addition, the leakage of the cascade process was reduced by exploring factors such as concentration and DNA structure. The proposed DNAzyme activity regulation strategy provides great potential for the expansion of logic circuits in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据