A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

This work reports on a modularized electrochemical method for the determination of the hormones cortisol, progesterone, testosterone and 17 beta-estradiol in urine. These hormones were employed as templates when generating molecular imprints from aniline and metanilic acid by electropolymerization on the surface of screen-printed electrodes. The electrically conductive imprint was characterized by SEM, AFM and cyclic voltammetry. A four-channel system was then established to enable simultaneous determination of the hormones by cyclic voltammetry. The detection limits for cortisol, progesterone, testosterone and 17 beta-estradiol are as low as 2, 2.5, 10 and 9 ag center dot mL(-1) (for S/N = 3).