期刊
MICROBIAL DRUG RESISTANCE
卷 26, 期 4, 页码 353-359出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/mdr.2019.0249
关键词
Klebsiella pneumoniae; fruits and vegetables; 3rd-generation cephalosporin (3GC) resistance; whole genome sequencing; Algeria
资金
- French Ministry for Agriculture
- Algerian Ministry of Higher Education and Scientific Research
This study aimed to characterize third-generation cephalosporin (3GC)-resistant Klebsiella pneumoniae isolated from fresh fruits and vegetables purchased at Bejaia city, Algeria. K. pneumoniae isolates were identified by MALDI-TOF. Susceptibility to antibiotics was tested by the disk diffusion method. Whole genome sequencing (WGS) was carried out to determine sequence type (ST), plasmid incompatibility group (Inc.), and acquired antimicrobial resistance gene presence. A total of 13 3GC-resistant K. pneumoniae strains were isolated. WGS identified bla(CTX-M-15) in 11 extended-spectrum-beta-lactamases (ESBL)-K. pneumoniae and bla(DHA-1) in 2 AmpC-K. pneumoniae. The aac(6')lb-cr gene was identified in 8 out of 13 isolates. Multilocus sequence typing (MLST) evidenced five different STs, namely ST14, ST45, ST219, ST236, and ST882. MDR K. pneumoniae contaminated fresh fruits and vegetables, often eaten raw and inappropriately washed, may represent an underestimated public health threat. This study highlights that hygiene measures during harvesting and retail process are of utmost importance to limit further ESBL/AmpC spread to the consumer households.
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