期刊
BIOPHYSICAL JOURNAL
卷 110, 期 7, 页码 1469-1475出版社
CELL PRESS
DOI: 10.1016/j.bpj.2016.02.032
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资金
- National Institutes of Health [R01 GM072004, GM0884905]
- National Science Foundation [CMMI 1361987]
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1361987] Funding Source: National Science Foundation
The application of flow visualization in biological systems is becoming increasingly common in studies ranging from intracellular transport to the movements of whole organisms. In cell biology, the standard method for measuring cell-scale flows and/or displacements has been particle image velocimetry (PIV); however, alternative methods, exist, such as optical flow constraint. Here we review PIV and optical flow, focusing on the accuracy and efficiency of these methods in the context of cellular biophysics. Although optical flow is not as common, a relatively simple implementation of this method can outperform PIV and is easily augmented to extract additional biophysical/chemical information such as local vorticity or net polymerization rates from speckle microscopy.
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