期刊
MARINE DRUGS
卷 17, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/md17110606
关键词
hybrid polyketides; tetramic acid; Cladosporium sphaerospermum; hybrid PKS-NRPS; LLC-PK1 cells
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIT) [2018R1A2B2006879, 2019R1A5A2027340]
- Nano Convergence Industrial Strategic Technology Development Program - Ministry of Trade, Industry & Energy (MOTIE, Korea) [20000105]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20000105] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Five hybrid polyketides (1a, 1b, and 2-4) containing tetramic acid core including a new hybrid polyketide, cladosin L (1), were isolated from the marine fungus Cladosporium sphaerospermum SW67, which was isolated from the marine hydroid polyp of Hydractinia echinata. The hybrid polyketides were isolated as a pair of interconverting geometric isomers. The structure of 1 was determined based on 1D and 2D NMR spectroscopic and HR-ESIMS analyses. Its absolute configuration was established by quantum chemical electronic circular dichroism (ECD) calculations and modified Mosher's method. Tetramic acid-containing compounds are reported to be derived from a hybrid PKS-NRPS, which was also proved by analyzing our C-13-labeling data. We investigated whether compounds 1-4 could prevent cell damage induced by cisplatin, a platinum-based anticancer drug, in LLC-PK1 cells. Co-treatment with 2 and 3 ameliorated the damage of LLC-PK1 cells induced by 25 mu M of cisplatin. In particular, the effect of compound 2 at 100 mu M (cell viability, 90.68 +/- 0.81%) was similar to the recovered cell viability of 88.23 +/- 0.25% with 500 mu M N-acetylcysteine (NAC), a positive control.
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