期刊
MARINE DRUGS
卷 17, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/md17120663
关键词
Streptomyces sp. SCSIO 40010; marine; genome mining; polycyclic tetramate macrolactams; cytotoxicity
资金
- NSFC [31630004, 41606193]
- Administration of Ocean and Fisheries of Guangdong Province [A201601C03]
- Science and Technology Program of Guangzhou [201707010181]
- Chinese Academy of Sciences [QYZDJ-SSW-DQC004]
- Qingdao National Laboratory for Marine Science and Technology [QNLM2016ORP0304]
Polycyclic tetramate macrolactams (PTMs) biosynthetic gene cluster are widely distributed in different bacterial types, especially in Streptomyces species. The mining of the genomic data of marine-derived Streptomyces sp. SCSIO 40010 reveals the presence of a putative PTM-encoding biosynthetic gene cluster (ptm ' BGC) that features a genetic organization for potentially producing 5/5/6 type of carbocyclic ring-containing PTMs. A fermentation of Streptomyces sp. SCSIO 40010 led to the isolation and characterization of six new PTMs 1-6. Comprehensive spectroscopic analysis assigned their planar structures and relative configurations, and their absolute configurations were deduced by comparing the experimental electronic circular dichroism (ECD) spectra with the reported spectra of the known PTMs. Intriguingly, compounds 1-6 were determined to have a trans-orientation of H-10/H-11 at the first 5-membered ring, being distinct from the cis-orientation in their known PTM congeners. PTMs 1-5 displayed cytotoxicity against several cancer cell lines, with IC50 values that ranged from 2.47 to 17.68 mu M.
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