期刊
MARINE DRUGS
卷 17, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/md17100593
关键词
genome sequencing; gene disruption; lobophorin; metabolic engineering; genome mining
资金
- National Natural Science Foundation of China [81425022, U1706206, U1501223]
- Natural Science Foundation of Guangdong Province [2016A030312014]
Marine-sourced actinomycete genus Streptomyces continues to be an important source of new natural products. Here we report the complete genome sequence of deep-sea-derived Streptomyces olivaceus SCSIO T05, harboring 37 putative biosynthetic gene clusters (BGCs). A cryptic BGC for type I polyketides was activated by metabolic engineering methods, enabling the discovery of a known compound, lobophorin CR4 (1). Genome mining yielded a putative lobophorin BGC (lbp) that missed the functional FAD-dependent oxidoreductase to generate the D-kijanose, leading to the production of lobophorin CR4 without the attachment of D-kijanose to C17-OH. Using the gene-disruption method, we confirmed that the lbp BGC accounts for lobophorin biosynthesis. We conclude that metabolic engineering and genome mining provide an effective approach to activate cryptic BGCs.
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