期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 70, 期 3, 页码 130-136出版社
OXFORD UNIV PRESS
DOI: 10.1111/lam.13252
关键词
antibiotic resistance; calves; Escherichia coli phylo-type; foals; lambs
资金
- Foundation for Research Support of the State of Rio Grande do Sul (Brazil) [68.902.901.582.806.000.000, FAPERGS/CAPES 17/2012]
- Coordination of Improvement of Higher-Level Personnel (Brazil) [2734/2011]
Data about phylogenetic classification of Escherichia coli colonizing calves, lambs and foals are routinely neglected and restricted to outdated methodologies, even in the context of antimicrobial susceptibility (AS) testing. Thus, the aim of this study was to determine the phylogenetic diversity and the AS profile of E. coli colony-forming units (CFUs) from faecal samples of healthy animals. Five CFUs of E. coli were randomly selected from each faecal culture of calves (n = 13), foals (n = 13) and lambs (n = 13), totalizing 195 CFUs phylo-typed by quadruplex PCR. The AS profile of five CFUs from 15 samples (five from each animal species; n = 75 isolates) against nine drugs was determined by agar diffusion test. We found E. coli belonging to all phylo-groups already described, except D group, with the predominance of B1 (65% CFUs; 126/195) in the three-animal species sampled. Most faecal samples of calves (77%; 10/13) and foals (69%; 9/13) harboured both pathogenic and nonpathogenic E. coli. All faecal samples showed CFUs with diverse AS profile, highlighting the ineffectiveness of tetracycline, sulphonamide and ampicillin. As a key point, our data reinforce the importance to select at least four E. coli CFUs for AS testing.
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