期刊
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
卷 100, 期 5, 页码 1860-1866出版社
WILEY
DOI: 10.1002/jsfa.10074
关键词
nitroxynil; monoclonal antibody; immunochromatographic strip; rapid test
资金
- National Key Research and Development Program of China [2018YFC1602903]
- National Science & Technology Pillar Program of '12th Five-Year Plan' [2014BAD13B05]
BACKGROUND Nitroxynil (NIT) is a veterinary drug against hepatic fluke disease for food-producing cattle and sheep. NIT has a long half-life time in animals since it is highly bound to plasma protein. Therefore NIT possibly remains in animal edible tissues or milk due to drug abuse. In this study, a specific murine monoclonal antibody (mAb) against NIT was prepared and an immunochromatographic strip assay based on the mAb was developed for screening NIT in milk. RESULTS The affinity constant of the anti-NIT mAb was 2.93 x 10(10) and the anti-NIT mAb had almost no cross-reactivity with other analogs, so that it showed good specificity. The cutoff value of this test strip was considered to be 50 ng mL(-1) by the naked eye. When detected by the strip reader, the half maximum inhibition concentration (IC50) of the immunoassay strip was calculated to be 5.716 ng mL(-1) and the limit of detection (LOD) was 1.146 ng mL(-1). Intra-assay recoveries from 88.80 to 97.13% were obtained, with the highest coefficient of variation (CV) at 9.01%; inter-assay recoveries ranged from 84.60 to 106.87%, with the highest CV at 9.93%. CONCLUSION The operative procedure of the proposed method can be completed within 10 min. The strip developed in this study was a practical tool for rapid semiquantitative and quantitative detection of NIT in milk. This study suggested great potential for analytically monitoring NIT in other food samples. (c) 2019 Society of Chemical Industry
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据