期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 141, 期 47, 页码 18644-18648出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b07632
关键词
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资金
- European Union [746087]
- Scientific Center for Optical and Electron Microscopy (ScopeM) of ETH Zurich
- Marie Curie Actions (MSCA) [746087] Funding Source: Marie Curie Actions (MSCA)
Bioorthogonal reactions are valuable tools for the selective labeling and imaging of natural products and proteins. Here, we present the reaction between isonitriles and chlorooximes as a ligation that proceeds quickly (k approximate to 1 M-1 s(-1)) and with high chemoselectivity in an aqueous environment. Imaging of metabolically labeled cell surface glycans underlined the tolerance of the ligation to common functional groups in cellular systems. Live-cell dual-labeling experiments revealed that the isonitrile-chlorooxime ligation is orthogonal to the strain-promoted azide-alkyne cycloaddition.
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