期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 141, 期 42, 页码 16997-17005出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b09571
关键词
-
资金
- Eastman Kodak fellowship
- NIH [5R21GM129879-02, 1DP2AI15207201, 1DP2A115207201]
- DOE Photonics at Thermodynamic Limits Energy Frontier Research Center [DE-SC0019140]
- Stanford Bio-X Interdisciplinary Initiatives Committee (IIP)
- Gordon and Betty Moore Foundation [4309]
- Stanford Neurosciences Institute [119600]
- National Institutes of Health [1R01GM128089-01A1]
- Stanford Cancer Translational Nanotechnology Training Grant - National Cancer Institute [T32 CA196585]
- Photonics at Thermodynamic limits Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC0019140]
- National Science Foundation [ECCS-1542152]
- NSF GRFP [2013156180]
Upconverting nanoparticles provide valuable benefits as optical probes for bioimaging and Forster resonant energy transfer (FRET) due to their high signal-to-noise ratio, photostability, and biocompatibility; yet, making nanoparticles small yields a significant decay in brightness due to increased surface quenching. Approaches to improve the brightness of UCNPs exist but often require increased nanoparticle size. Here we present a unique core-shell-shell nanoparticle architecture for small (sub-20 nm), bright upconversion with several key features: (1) maximal sensitizer concentration in the core for high near-infrared absorption, (2) efficient energy transfer between core and interior shell for strong emission, and (3) emitter localization near the nanoparticle surface for efficient FRET. This architecture consists of beta-NaYbF4 (core) @NaY0.8-xErxGd0.2F4 (interior shell) @NaY0.8Gd0.2F4 (exterior shell), where sensitizer and emitter ions are partitioned into core and interior shell, respectively. Emitter concentration is varied (x = 1, 2, 5, 10, 20, 50, and 80%) to investigate influence on single particle brightness, upconversion quantum yield, decay lifetimes, and FRET coupling. We compare these seven samples with the field-standard core-shell architecture of beta-NaY0.58Gd0.2Yb0.2Er0.02F4 (core) @NaY0.8Gd0.2F4 (shell), with sensitizer and emitter ions codoped in the core. At a single particle level, the core-shell-shell design was up to 2-fold brighter than the standard core-shell design. Further, by coupling a fluorescent dye to the surface of the two different architectures, we demonstrated up to 8-fold improved emission enhancement with the core-shell-shell compared to the core-shell design. We show how, given proper consideration for emitter concentration, we can design a unique nanoparticle architecture to yield comparable or improved brightness and FRET coupling within a small volume.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据