期刊
JOURNAL OF PROTEOME RESEARCH
卷 18, 期 12, 页码 4085-4097出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00503
关键词
plasma; serum; blood; Human Proteome Project (HPP); Human Plasma Proteome Project (HPPP); study design; sample collection; quality metrics; plasma processing workflows; data acquisition; mass spectrometry (MS); data processing; bioinformatic analysis
资金
- National Institutes of Health [R0IGM087221, R24GM127667, U19AG02312, U54ES017885, U24CA210967-01]
- KTH Center for Applied Precision Medicine - Erling-Persson Family Foundation Grant for Science for Life Laboratory
- Knut and Alice Wallenberg Foundation
- Max Planck Society for the Advancement of Science
- Novo Nordisk Foundation [NNF15CC0001]
The proteomic analysis of human blood and blood-derived products (e.g., plasma) offers an attractive avenue to translate research progress from the laboratory into the clinic. However, due to its unique protein composition, performing proteomics assays with plasma is challenging. Plasma proteomics has regained interest due to recent technological advances, but challenges imposed by both complications inherent to studying human biology (e.g., interindividual variability) and analysis of biospecimens (e.g., sample variability), as well as technological limitations remain. As part of the Human Proteome Project (HPP), the Human Plasma Proteome Project (HPPP) brings together key aspects of the plasma proteomics pipeline. Here, we provide considerations and recommendations concerning study design, plasma collection, quality metrics, plasma processing workflows, mass spectrometry (MS) data acquisition, data processing, and bioinformatic analysis. With exciting opportunities in studying human health and disease though this plasma proteomics pipeline, a more informed analysis of human plasma will accelerate interest while enhancing possibilities for the incorporation of proteomics-scaled assays into clinical practice.
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