期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 200, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2019.111654
关键词
Photodynamic therapy; Microbicide; Phenothiazine; Enterococcus spp
资金
- Brazilian National Council for Scientific and Technological Development - CNPq
- Bahia State Research Support Foundation FAPESB
- Coordination of Superior Level Staff Improvement CAPES
The Enterococcus faecalis is a microorganism that causes multiple forms of resistance to a wide range of drugs used clinically. aPDT is a technique in which a visible light activates photosensitizer (PS), resulting in generation of reactive oxygen species that kill bacteria unselectively via an oxidative burst. aPDT is an alternative to antibiotics with the advantage of not causing resistance. The search for an alternative treatment of infections caused by E. faecalis, without using antibiotics, is off great clinical importance. The aim of present investigation was to assess the efficacy of using 3.32 eta g/mL of 1,9-dimethylmethylene blue (DMMB) as photosensitizer associated with the use of either Laser (lambda 660 nm) or LED (lambda 632 +/- 2 nm) using different energy densities (6, 12 and 18 J/cm(2)) to kill E. faecalis in vitro. Under different experimental conditions, 14 study groups, in triplicate, were used to compare the efficacy of the aPDT carried out with either the laser or LED lights using different energy densities associated to DMMB. The most probable number method (MPN) was used for quantitative analysis. Photodynamic antimicrobial effectiveness was directly proportional to the energy density used, reaching at 18 J/cm(2), 99.999998% reduction of the counts of E. faecalis using both light sources. The results of this study showed that the use of 3.32 eta g/mL of DMMB associated with the use 18 J/cm(2) of LED light (lambda 632 +/- 2 nm) reduced > 7-log counts of planktonic culture of E. faecalis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据