Expression of FOXP3 in Canine Gliomas: Immunohistochemical Study of Tumor-Infiltrating Regulatory Lymphocytes

Dogs develop gliomas with similar histopathological features to human gliomas and share with them the limited success of current therapeutic regimens such as surgery and radiation. The tumor microenvironment in gliomas is influenced by immune cell infiltrates. The present study aims to immunohistochemically characterize the tumor-infiltrating lymphocyte (TIL) population of naturally occurring canine gliomas, focusing on the expression of Forkhead box P3-positive (FOXP3(+)) regulatory T-cells (Tregs). Forty-three canine gliomas were evaluated immunohistochemically for the presence of CD3(+), FOXP3(+), and CD20(+) TILs. In low-grade gliomas, CD3(+) TILs were found exclusively within the tumor tissue. In high-grade gliomas, they were present in significantly higher numbers through-out the tumor and in the brain-tumor junction. CD20(+) TILs were rarely found in comparison to CD3(+) TILs. FOXP3(+) TILs shared a similar distribution with CD3(+) TILs. The accumulation of FOXP3(+) Tregs within the tumor was more pronounced in astrocytic gliomas than in tumors of oligodendroglial lineage and the difference in expression was significant when comparing low-grade oligodendrogliomas and high-grade astrocytomas. Only high-grade astrocyto mas presented FOXP3(+) cells with tumoral morphology. In spontaneous canine gliomas, TILs display similar characteristics (density and distribution) as described for human gliomas, supporting the use of the dog as an animal model for translational immunotherapeutic studies.