期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 476, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jim.2019.112679
关键词
Next-generation sequence; TCR beta; Neoantigen; Cancer immunotherapy
资金
- Japan Society for the Promotion of Science (JSPS KAKENHI) [JP:15H04761, 16H06277]
- Shiga Prefecture (Japan)
- IMSUT (Institute of Medical Sciences, The University of Tokyo)
Development of mechanism-driven biomarkers for immune checkpoint inhibitors (ICIs) in cancer immunotherapy requires sensitive and efficacious assays to identify tumor antigen (Ag)-specific T cells. We demonstrated the concept for a sensitive method to determine Ag-reactive T cell clones based on clonal expansion using model neoantigens (NeoAgs) rather than cytokine production. Sequential increase in T cell clonal frequencies following Ag stimulation was detected by next generation sequencing (NGS) of T cell receptor beta (TCR beta) complementarily-determining region 3 (CDR3), with a higher sensitivity than that of enzyme-linked immunospot (ELISPOT) by 100-fold. The TCR beta CDR3 sequences could represent useful markers to track dynamic changes during immunotherapy. The TCR beta NGS-based method could represent a novel platform both for the development of new biomarkers as well as several therapeutic options.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据