4.7 Article

Formation of di-cysteine acrolein adduct decreases cytotoxicity of acrolein by ROS alleviation and apoptosis intervention

期刊

JOURNAL OF HAZARDOUS MATERIALS
卷 387, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.jhazmat.2019.121686

关键词

ACR-Cys adduct; Cytotoxicity; ROS; Apoptosis; Free cysteine

资金

  1. National Natural Science Foundation of China [31871902]
  2. Guangzhou Municipal Science and Technology Project [201903010004]

向作者/读者索取更多资源

Acrolein (ACR) is a toxic contaminant for humans. Our previous research indicated that L-cysteine (Cys) decreased the cytotoxicity of acrolein possibly via adduct formation, but which adduct contributed to the toxicity-lowering effect remains unknown. In this work, we identified a di-cysteine acrolein adduct (ACR-di-Cys) and investigated its toxicity against human bronchial epithelial cell line HBE and colon cancer cell line Caco-2. ACR-di-Cys tremendously decreased acrolein-induced cytotoxicity via alleviating ROS and apoptosis intervention. In the condition of no presence of free cysteine, however, this adduct can convert to mono-ACR-Cys in PBS solution by losing a molecule of cysteine conjugated at C=C bond. ACR-mono-Cys showed much higher toxicity than ACR-di-Cys, and even higher than acrolein after 48h exposure. This study indicated that cysteine can react with acrolein to form adducts with different acrolein-detoxifying capacity, and a sufficient intake of cysteine or cysteine-containing proteins can maximize the detoxifying effect for acrolein via the formation of a highly detoxifying agent, ACR-di-Cys.

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