Cyanidin-3-O-glucoside restores spermatogenic dysfunction in cadmium-exposed pubertal mice via histone ubiquitination and mitigating oxidative damage

Cadmium (Cd) is an environmental contaminant found in soil, water, and food, and can cause oxidative stress and male reproductive damage. During puberty, the male reproductive system is very vulnerable to interference, however, the dysregulation of Cd on spermatogenesis in this period is ambiguous. The anthocyanin cyanidin-3-O-glucoside (C3G) is phytochemical rich in plants and fruits and has been shown to have remarkable antioxidant activity, making it an ideal nutrient for nutritional intervention. By modeling Cd-induced damage in male pubertal mice and feeding with C3G, we demonstrated that the C3G could rescue the amount and activity of sperm predominantly. Furthermore, C3G showed partial resistance to Cd-induced histone modification during spermiogenesis and prevented oxidative damage of the DNA in the sperm nucleus. Additionally, C3G mitigated the oxidative stress of testis to achieve the level coinciding with the control group. Meanwhile, Cd-induced mitochondrial apoptosis of sperm cells was reduced significantly via the MAPK signaling pathway in the presence of C3G. Collectively, our findings can offer a potential intervention for combating Cd-induced reproductive damage during puberty by taking anthocyanin as a dietary supplement.