期刊
JOURNAL OF DAIRY SCIENCE
卷 102, 期 11, 页码 9570-9585出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2019-16840
关键词
naturally fermented pickle; Lactobacillus fermentum; dextran sulfate sodium (DSS); colitis; inflammatory factor
资金
- Foundation of Chongqing Science and Technology Bureau [cstc2017shms-kjfp80011, cstc2017shms-kjfp80053]
- Venture and Innovation Support Program for Chongqing Overseas Returnees [cx2018095]
- Fundamental Research Funds for the Central Universities [XDJK2017B039]
Colitis severely affects the quality of life of patients, and lactic acid bacteria have been reported to be able to improve or treat colitis. In this study, we selected a strain of Lactobacillus fermentum (CQPC04) with good resistance in vitro to evaluate its effect on improvement in mice with dextran sulfate sodium (DSS)-induced colitis. We analyzed the effects of L. fermentum CQPC04 on mice with colitis macroscopically via colon length and histopathology. We also used conventional biochemical and ELISA kits, real-time quantitative PCR (RT-qPCR), and Western blotting to analyze microscopically the effects of L. fermentum CQPC04 on related oxidant indices and pro- and anti-inflammatory cytokines in serum and colon tissue of mice. The results indicated that L. fermentum CQPC04 notably increased colon length and ameliorated pathological damage of colon tissue in colitic mice. Serum indices showed that L. fermentum CQPC04 increased the enzyme activity of total superoxide dismutase (T-SOD) and catalase (CAT) and decreased the content of malondialdehyde (MDA) and the activity of myeloperoxidase (MPO). In addition, it inhibited the release of the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), IFN-gamma, IL-1 beta, IL-6, and IL-12, and increased the release of the anti-inflammatory cytokine IL-10 in serum. The RT-qPCR experiments confirmed that L. fermentum CQPC04 downregulated. the expression of pro-inflammatory cytokine nuclear factor-kappa B-p65 (NF-kappa Bp65), NF-kappa B inhibitor-alpha (I kappa B-alpha), TNF-alpha, IFN-gamma, IL-1 beta, IL-6, cyclooxygenase 2 (COX-2), and inducible nitric oxide synthase (iNOS), and upregulated the expression of IL-10 in colon tissue. Western blot analysis indicated that L. fermentum CQPC04 significantly reduced expression of NF-kappa Bp65, TNF-alpha, IL-1 beta, COX-2, and iNOS in mouse colon tissues, and increased expression of I kappa B-alpha and superoxide dismutase 2 (SOD2). Thus, L. fermentum CQPC04 could effectively alleviate the symptoms of DSS-induced colitis mice and is a potential probiotic for human experiments.
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