期刊
JOURNAL OF DAIRY SCIENCE
卷 102, 期 11, 页码 9663-9673出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2019-16292
关键词
antibacterial activity; Litsea mollis Hemsl. essential oil; mechanism of action; chemical composition
资金
- Natural Science Foundation of Zhejiang Province (Hangzhou, China) [LY18C06004]
- National Natural Science Foundation of China (Beijing) [31871775]
Chemical preservatives have potential safety hazards, which may pose threats to human health. Safer biopreservatives are therefore urgently required. This study investigated the bacteriostatic activity arid mechanism of Litsea mollis Hemsl. essential oil against Escherichia coli DH5 alpha and Salmonella spp. Antibacterial activity of Litsea mollis Hemsl. essential oil 9 (LMEO9) against E. coli DH5 alpha was observed (zone of inhibition was 5.0 +/- 0.2 mm; minimum inhibitory concentration was 0.05%). Increases in electrolyte, nucleic acid, and alkaline phosphatase leakage in LMEO9-treated bacteria suggested that the cell envelope had been damaged. Scanning and transmission electron microscopy also demonstrated morphological alterations and content leakage during LMEO9 treatment. According to the kill-time analysis arid propidium iodide uptake assay, LMEO9 led to cell death. These results demonstrated that LMEO9, which could affect bacterial cell envelope structural integrity, is a low-cost biopreservative that could be useful for the dairy industry and in fresh storage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据